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. 2017 Mar 28;9(4):62. doi: 10.3390/v9040062

Figure 2.

Figure 2

Exchanging the core gene between the two HBV genotypes did not alter the intracellular levels of replicative DNA. The core gene was exchanged between clones of the two HBV genotypes, as both 1.1mer construct (left panels) and dimer construct (right panels). Chimeras with the envelope gene exchanged were analyzed in parallel for comparison. Huh7 cells were transiently transfected with these HBV constructs and harvested four days later. (A) Southern blot analysis of replicative DNA from cell lysate using mixed probes of the two genotypes. A genotype A (clone 4B) mutant deficient in pregenomic RNA (pgRNA) encapsidation (ε−) served as a negative control. (B) Secreted HBsAg averaged from three transfection experiments (1:300 dilution for 1.1mer, 1:500 for dimer), with values for clone 22.5 set arbitrarily at 100.