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. 2017 Mar 28;9(4):62. doi: 10.3390/v9040062

Figure 4.

Figure 4

Effect of exchanging two codons in the preS1 region between the two HBV genotypes on the efficiency of virion secretion. Codons 108 and 115 in the preS1 region were exchanged between the 1.1mer constructs of genotype B and genotype C clones. Huh7 cells were transiently transfected with the parental constructs and site-directed mutants, and harvested four days later. Southern blot analysis of (A) intracellular replicative DNA and (B) virion DNA using mixed genotype B/C probes. (C) Calculated ratio of extracellular virion DNA/intracellular replicative DNA following densitometric analysis of the Southern blots from three independent transfection experiments. The value for clone 22.5 of genotype B was set arbitrarily at 1. (D) Secreted HBsAg values from three independent transfection experiments, with the value for clone 22.5 set arbitrarily at 100. Culture supernatant was diluted 1:300 for ELISA.