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. 2017 Mar 28;9(4):62. doi: 10.3390/v9040062

Figure 5.

Figure 5

Impact of exchanging codon 126 in the S region between genotype B and genotype C clones on HBV virion secretion. The T126I mutation was introduced to two genotype B clones, while the I126T mutation was introduced to two genotype C clones. The parental constructs and the site-directed mutants as 1.1mer construct (left) or SphI dimer (right) were transiently transfected to Huh7 cells, which were harvested 4 days later. Shown are Southern blot analysis of (A) intracellular replicative DNA and (B) virion-associated DNA, (C) the ratio of extracellular virion DNA/intracellular replicative DNA as an indicator of secretion efficiency, (D) and secreted HBsAg. Culture supernatant was diluted 1:300 (for 1.1mer) or 1:500 (for dimer) for ELISA.