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. 2017 Apr 3;9(4):71. doi: 10.3390/v9040071

Figure 1.

Figure 1

Detection of Marek’s disease virus (MDV) by polymerase chain reaction (PCR). DNA of MDV-infected chicken embryo fibroblasts (CEFs) was used as the template for PCR amplification. (A) PCR amplification of the meq gene of MDV. The PCR products of BS/15 and Md5 were 1403 base pair (bp) long, while those of CVI988 and 814 were 1580 bp, as they have a 177-bp insertion in the meq gene; (B) PCR amplification of the 132bpr of MDV. The PCR product of BS/15 132bpr was 448 bp long with a copy number of 3, while the PCR product length for Md5 was 316 bp with a copy number of 2, CVI988 and 814 showed PCR products 316–844 bp long and contained multiple copies of the 132bpr.