Figure 5.

In vitro translation of AUG2 mutational variants of Ty1i RNA and melting profile of AUG1AUG2 RNA. (A) In vitro transcribed capped or uncapped transcripts were translated using wheat germ extract in the presence of ³⁵S-methionine. Calculated translation activity (in relation to the capped AUG1AUG2 RNA) is shown below the gel. (B) Melting of AUG1AUG2 RNA followed by SHAPE at 37 °C and 60 °C. Nucleotides are coloured according to their reactivity (black, green, orange, red). The segment of domain I with the strongest changes at 60 °C is boxed.