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. 2017 Apr 10;9(4):74. doi: 10.3390/v9040074

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of the hairpin H1 deletion on Ty1i RNA, p22 protein expression and Ty1his3-AI mobility. (A) Northern blotting of total RNA from the 1 Ty1 strain (DG2196) and 0 Ty1 strain (DG3582) containing either wild type (WT) pGPOLΔ or mutant pH1Δ plasmids. A [³²P]-labeled Ty1 riboprobe (nt 1266 to 1601) was used to detect Ty1i RNA. ACT1 mRNA served as a loading control. Below are Ty1i:ACT1 ratios as determined by phosphorimaging. (B) Whole cell extracts from strains used in (A) were immunoblotted with p18 antiserum to detect p22. Pgk1 served as a loading control. p22:Pgk1 ratios were determined by densitometry. (C) Quantitative Ty1his3-AI mobility assayed in the 1 Ty1 strain containing one genomic Ty1his3-AI element and empty vector, WT, or H1Δ plasmids. All strains were grown in glucose containing medium to repress GAL1-promoted Ty1 expression. Bars denote standard deviation.