Table 1. Newly designed primers based on the nuclear small subunit ribosomal DNA (SSU rDNA) in this study.
| Primer name | Sequences (5′→3′) | Amplicon length (bp) | Ta (°C) |
|---|---|---|---|
| 18S286F | GTCCGCCCTCTGGGTG | 286 | 55 |
| 18S286R | TCGCAGTAGTSYGTCTTTAAC | ||
| 18S468F | GAAATAACAATACARGGCATCCAT | 468 | 59 |
| 18S468R | TTCGCAGTAGTCCGTCTTTAAC | ||
| 18S489F | TGGCCGTTCTTAGTTGGTG | 489 | 57 |
| 18S489R | TGTTACGACTTCTCCTTCCTCT | ||
| 18S493F | CATGGCYGTTCTTAGTTGGTG | 493 | 57 |
| 18S493R | TGTTACGACTTCTCCKTCCTCT | ||
| 18S634F | GGGTAACGGAGAATTAGGGTTT | 634 | 59 |
| 18S634R | TCCCCTAACTTTCGTTCTTGATC |