Table 2. Preliminary tests for all primers designed in this study based on single dinocysts.
Primers | Dinoflagellate species | |||||
---|---|---|---|---|---|---|
Gouyaulax spinifera | Lingulodinium polyedrum | Pyrophacus steinii | Polykrikos kofoidii | Protoperidinium leonis | Protoperidinium oblongum | |
18S286F–18S286R | Clear band | Clear band | Clear band | Clear band | Clear band | Clear band |
18S468F-18S468R | No band | − | − | − | No band | No band |
18S489F-18S489R | No band | No band | Faint band | No band | − | Clear band |
18S493F-18S493R | No band | − | No band | No band | Faint band | No band |
18S634F-18S634R | Clear band | Clear band | Clear band | Clear band | Clear band | Clear band |
Notes.
“−” indicates that primers were not tested for corresponding dinocysts due to the limited amount of isolated single dinocysts.