Table 3. Comparisons of effects on the molecular identification of single dioncysts by different cleaning methods.

Sample number	Cleaning methods	Morphology identification	Trophic type	Molecular identification	Success rate
1	Micropipette	Gouyaulax sp.	Plastid-containing	Failure	16.70%
2		Gouyaulax spinifera	Plastid-containing	Failure
3		Lingulodinium polyedrum	Plastid-containing	Failure
4		Pyrophacus steinii	Plastid-containing	Pyrophacus steinii
5		Polykrikos kofoidii	Heterotrophic	Failure
6		Polykrikos kofoidii	Heterotrophic	Polykrikos kofoidii
7		Polykrikos kofoidii	Heterotrophic	Failure
8		Protoperidinium leonis	Heterotrophic	Failure
9		Protoperidinium leonis	Heterotrophic	Failure
10		Protoperidinium oblongum	Heterotrophic	Failure
11		Protoperidinium oblongum	Heterotrophic	Failure
12		Protoperidinium oblongum	Heterotrophic	Failure
13	Optimized ultrasonic wave cleaning	Gouyaulax spinifera	Plastid-containing	Gouyaulax spinifera	86.70%
14		Gouyaulax sp.	Plastid-containing	Gonyaulax cochlea
15		Gouyaulax spinifera	Plastid-containing	Failure
16		Gouyaulax spinifera	Plastid-containing	Failure
17		Gouyaulax spinifera	Plastid-containing	Gouyaulax spinifera
18		Gouyaulax spinifera	Plastid-containing	Gouyaulax spinifera
19		Gouyaulax spinifera	Plastid-containing	Gouyaulax spinifera
20		Lingulodinium polyedrum	Plastid-containing	Lingulodinium polyedrum
21		Lingulodinium polyedrum	Plastid-containing	Lingulodinium polyedrum
22		Pyrophacus steinii	Plastid-containing	Pyrophacus steinii
23		Polykrikos kofoidii	Heterotrophic	Polykrikos kofoidii
24		Polykrikos kofoidii	Heterotrophic	Polykrikos kofoidii
25		Polykrikos kofoidii	Heterotrophic	Polykrikos kofoidii
26		Protoperidinium claudicans	Heterotrophic	Protoperidinium claudicans
27		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
28		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
29		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
30		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
31		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
32		Protoperidinium leonis	Heterotrophic	Failure
33		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
34		Protoperidinium leonis	Heterotrophic	Protoperidinium leonis
35		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum
36		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum
37		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum
38		Protoperidinium oblongum	Heterotrophic	Failure
39		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum
40		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum
41		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum
42		Protoperidinium oblongum	Heterotrophic	Protoperidinium oblongum