Figure 3. Light and fluorescence microscopy images using PI and YP fluorescent dye assays.

(A, B, G, H) untreated control; (C, D, I, J) cells after the 50 × 4, 5.5 T pulsing protocol; (E, F, K, L) cells after the single 100 µs, 1.2 kV/cm pulsing protocol. Arrows are used to highlight the cells with weak mean fluorescence, which were counted as PI and YP positive during analysis.