Table 2. Degenerate primers used to clone 8 candidate reference genes for RT-qPCR in Amorphophallus.
Candidate reference genes | Forward primer sequence [5′–3′] | Reverse primer sequence [5′–3′] |
---|---|---|
EF1-a | GACTGCCACACCTSMCAYA | CKACRCTCTTGATRACACCR |
TUB | CGCCCYGACAACTTCGTCTT | CTTGGMRTCCCACATCTGCTG |
ACTB | AYGACATGGAGAARATYTGGC | CACCAGARTCMARCACAATAC |
H3 | SGTSAAGAAGCCCCACC | RCGRGCAAGCTGGATGT |
CYP | GAGAACTTCMGGGCNCTC | AYCTGSCCGAACACGACG |
GAPDH | CAADGACAAGGCTGCDGCTCA | CTTKGCDGCACCAGTGCTGC |
EIF4A | GATGAGCTNACCCTTGAGGGT | GCTGDACATCAATACCACGAGC |
UBQ | TRACGGGBAAGACCATCACN | ACCTTGTARAACTGGAGGASDGCG |