Table 4. Description of 9 candidate reference genes and SHSP gene in Amorphophallus.

Tm represents melt temperature and was calculated by the Bio-Rad CFX96 Real-Time PCR system.

Name	Forward primer sequence [5′–3′]	Reverse primer sequence [5′–3′]	Amplicon length (bp)	Tm (°C)	E (%)	R2
RP	GGACGAAGAGCAATGACCC	ACCCTTTCCCCGAACCCA	118	79.5	92.8	0.991
EF1-a	AAGTTCCTGAAGAATGGCGAT	GTCCCTCACGGCAAACCTACC	111	82.5	99.8	0.990
TUB	GCTGGTTGAGAATGCCGATGAA	GCAGAAATAAGGTGATTGAGAT	120	80	97.7	0.998
ACTB	CCAACAGAGAGAAGATGACA	ACCAGAATCCAGCACAATAC	128	79	94.3	0.999
H3	CGGGAGATCGCTCAGGACT	CATGATGGTGACGCGCTTG	139	86.5	96.8	0.990
CYP	CAAGCCCCTCCACTACAAGG	CCGGTGTGCTTCTTCACGAA	153	86	93.0	0.991
GAPDH	ACTAACTGCCTCGCTCCTC	CAGCCCTTCCACCCCTCCA	145	82	93.3	0.990
EIF4A	ACAAGATGAGGAGCAGGG	GGTGATAAGGACACGAGA	116	79.5	109.5	0.990
UBQ	GGACACCATCGACAACGTGA	TTCTTCTTGCGCTTCTTGGC	189	87.5	107.7	0.997
SHSP	ATCAAGGTCCAGGTGGAGGA	GGCAGCGAGAACTTCCTCAT	131	88	96.1	0.996