Fig. 2.

Characterization of scaffold architecture. (A) Fluorescently labeled PCL (red) and gelatin (green) displayed a fibrous morphology and interspersed distribution within the dry composite scaffold prepared by dual electrospinning (Composite Dry). Wetting of the scaffold with an aqueous solution resulted in dissolution of the gelatin fibers (green), whereas the PCL fibers (red) remained intact (Composite Wet). PCL fibers alone showed no change with wetting. (B) SEM revealed distinct microscopic architecture of the composite scaffold, in which ribbon-like bands were seen in addition to the predominant cylindrical fibers (Composite Dry, indicated by arrow in insert). After wetting, adjacent fibers were bridged or wrapped with a sheath of gelatin (Composite Wet, indicated by arrows in insert). Unlike the composite mesh, scaffolds made of only PCL showed negligible differences in morphology before and after wetting (PCL Dry vs. PCL Wet).