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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: Acta Biomater. 2016 Mar 2;35:68–76. doi: 10.1016/j.actbio.2016.03.004

Fig. 5.

Fig. 5

Preparation and characterization of a multilayer construct. (A) Scaffold sheets were wetted, stacked and exposed to VL for crosslink formation between adjacent scaffold layers to create a complex multi-layered structure. (B) Picrosirius red staining showed pronounced difference in scaffold integrity after washing among the three groups: stacked composite scaffold without crosslinking (CN, left), sandwich construct made from alternative layers of fibrous PCL and mGLT (PC, center), and crosslinked composite scaffold (CC, right). (C) Crosslinked scaffolds showed higher tensile strength than non-crosslinked scaffolds (CC vs. CN); the integration between fibrous scaffolds had little impact on overall tensile strength (CC vs. PC). *, p<0.05; n=6.