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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: Acta Biomater. 2016 Mar 2;35:68–76. doi: 10.1016/j.actbio.2016.03.004

Fig. 6.

Fig. 6

Analysis of cell distribution and metabolic activity in multilayer constructs. (A) Ethidium homodimer-1 (EthD-1, red) staining indicated that most cells were localized between adjacent layers. Crosslinked composite constructs (CC) demonstrated evidently higher structural integrity than crosslinked sandwich constructs (PC). (B) MTS assay revealed significantly higher metabolic activity of cells impregnated in composite scaffold (CC), whereas the total cell number remained comparable to that of sandwich constructs (PC). *, p<0.05; n=8.