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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: Acta Biomater. 2016 Mar 2;35:68–76. doi: 10.1016/j.actbio.2016.03.004

Fig. 7.

Fig. 7

Tendon-like features in cell-impregnated multilayer constructs. (A) Fiber alignment observed by SEM. (B) Elongated morphology of human adipose stem cells (hASCs) aligned in the direction of fibers (green, F-actin; blue, nuclei). (C) Anisotropy based on tensile strength properties measured by mechanical testing along two directions (Longi. vs. Cross). (D) Significant upregulation of tendon markers scleraxis (SCX) and tenascin C (TNC) upon treatment with exogenous tenogenic factor TGF-β3, measured by real-time PCR analysis.