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. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416

Figure 3. Characterization of the tetrathionate‐sensing TCS, TtrS/R (Sbal195_3859/8).

Figure 3

  1. Location of the thiosulfate sensor, consisting of TtrS (Sbal195_3859) and TtrR (Sbal195_3858), and tetrathionate reductase, TtrBCA (Sbal195_3860‐2), on the chromosome of Shewanella baltica OS195.
  2. Predicted domain architecture of TtrS and TtrR with the phosphotransfer residues indicated with an arrow. Domains are labeled by their Pfam family names. A scale bar is included for reference.
  3. Schematic of tetrathionate‐induced activation and plasmid design of the aTc‐ and IPTG‐inducible TtrSR components.
  4. Tetrathionate‐induced sfGFP production of the optimized truncated promoter PttrB185‐269 compared to the full‐length intergenic region PttrB344 in the presence (black bars) and absence (white bars) of 1 mM tetrathionate. The dotted horizontal line indicates cellular autofluorescence.
  5. Tetrathionate‐induced sfGFP production in the presence (black bars) and absence of 1 mM tetrathionate of wild‐type and inactivated sensors.
  6. Tetrathionate dose–response of the optimized promoter (closed circles).
  7. Selectivity of TtrS/R to tetrathionate over other terminal electron acceptors. All ligands were tested at 10 mM concentration.
Data information: Data are mean of at least three biological replicates ± SD.