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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: Cell Rep. 2017 Mar 21;18(12):2825–2835. doi: 10.1016/j.celrep.2017.02.071

Figure 2. SOX9+ cells contribute to alveologenesis and persist after repeated pregnancy.

Figure 2

(A) The experimental schedule for lineage-tracing studies shown in Figure 2.

(B) A representative tdTomato whole-mount image of Sox9-CreERT2; R26R-tdTomato mammary glands on day 17.5 of pregnancy (Preg17.5d).

(C) Percentages of tdTomato+ cells in the basal and luminal populations of Preg17.5d mice, as determined by flow cytometry (mean ± SEM, n = 3, paired t-test).

(D) K8 and K14 immunostaining of Preg17.5d mammary gland sections for characterizing the tdTomato+ cells. Examples of alveoli with either tdTomato-labeled luminal or basal cells were shown. The graph on the right shows percentage of alveoli containing the indicated types of tdTomato+ cells.

(E) ER immunostaining of Preg17.5d mammary gland sections. 99% tdTomato+ cells were ER−.

(F) A representative tdTomato whole-mount image of Sox9-CreERT2; R26R-tdTomato mammary glands at least 3 weeks after weaning of the second litter.

(G) Percentage of tdTomato+ cells in the basal, Sca1+ and Sca1− luminal populations 3 weeks after weaning of the second litter (mean ± SEM, n = 6).

(H) ER immunostaining of mammary glands after the 2nd pregnancy. 99.5% of tdTomato+ cells were ER−.

*P < 0.05 and **P < 0.01.

See also Figure S2.