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Volume fraction estimates for layers of various sample configurations in cell A. Volume fraction estimates were made based on the theoretical SLD values for the pure proteins at 90% H-D exchange. SLDs for pure rSPA and IgG were calculated using the Biomolecular Scattering Length Density calculator at http://psldc.isis.rl.ac.uk/Psldc/. Volume fraction estimates were calculated using Eq. (4).
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