Table 1.
80S/eIF5A–Hyp51 | 80S/eIF5A–Lys51 | |
---|---|---|
Data collection | ||
No. of crystals | 3 | 2 |
Space group | P21 | P21 |
Unit cell | ||
a, b, c (Å) | 435.63/286.45/303.41 | 438.23/289.33/305.47 |
α = β = 90°, γ(°) | 98.95 | 98.92 |
Resolution | 189.16–3.15 (3.25–3.15) | 190.48–3.25 (3.35–3.25) |
Rmeas | 36.0 (212.0) | 43.4 (235.6) |
I/σI | 6.54 (1.03) | 6.38 (0.98) |
CC1/2 | 99.4 (39.6) | 99.5 (35.5) |
Resolution at CC1/2 = 0.5 (Å) | 3.25 | 3.35 |
Completeness (%) | 99.9 (99.7) | 99.9 (99.9) |
Redundancy | 9.1 | 8.2 |
Refinement | ||
Resolution | 189.16–3.15 | 190.48–3.25 |
No. reflections | 1,261,726 | 1,176,370 |
Rwork/Rfree | 25.56/26.37 | 25.43/29.54 |
No. of atoms | 407,909 | 407,903 |
Protein | 184,678 | 185,006 |
RNA and ions | 223,231 | 220,897 |
B-factors | ||
Protein | 85.5 | 103.9 |
RNA and ions | 73.5 | 98.2 |
r.m.s. deviations | ||
Bond lengths (Å) | 0.006 | 0.006 |
Bond angles (Å) | 1.039 | 1.016 |
eIF5A-occupancy refinement (%) | 97 | 88 |
PDB ID | 5dat | 5dc3 |
Values in parenthesis show statistics of the highest resolution shell.