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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: Methods. 2016 Mar 25;105:99–108. doi: 10.1016/j.ymeth.2016.03.022

Table 1.

DNA and RNA sequences.

Sequences (5′ → 3′)
Pri-miRNA A: GAU ACU AUA CUG AGA GCA UUC CGU UAU GUA GCA UUU CUU GGU UGU GAG GGG UUG UGC
     Drosha-DGCR8 (Section 3.1) B: AAG AAG AAU CUC ACG AUC AAG GAA UGC UAC AU
     Strand C was Cy5-labeled C: AAC GGA GuG UUU GAG CAG ACC CGC GAC U
dsRNA D: AAG AAG AAU CUC ACG AUC AAG GAA UGC uAC AUA A
     Drosophila Dicer-2 (Section 3.2.1) E: pCGG AGU GUU UGA GCA GAC CCG CGA UCU UUC AUU GCC
     Strand D was Cy3-labeled F: CTC AAA CAC TCC GTT ATG TAG CAT TC
G: pGGC AAU GAA AGA UCG CGG GUC UGC UCA AAC ACU CCG UUA UGU AGC
H: pAUU CCU UGA UCG UGA GAU UCU UCU U
I: ACG ATC AAG GAA TGC TAC ATA ACG GA
pre-let-7a-1 J: UGA GGU AGU AGG UUG UAU AGU UUU AGG GUC ACA CC
     Human Dicer (Section 3.2.2) K: pCAC CAC UGG GAG AUA ACU AUA CAA UCU ACU GUC uUU CU
     Strand K was Cy5-labeled
Cy3-dA15 (section: 3.3) X: Cy3-AAA AAA AAA AAA AAA

p indicates phosphate; u represents dye-labeled nucleotide