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Antibodies used for immobilizing the proteins of interest.
| Antibodies | Final concentration | Reference |
|---|---|---|
| anti-FLAG antibody | 66 nM | F7425, Sigma |
| c-Myc antibody (A-14) | 66 nM | sc-789, Santa Cruz |
| Biotechnology | ||
| Biotin-SP (long spacer) AffinePure | 66 nM | 111-065-003, Jackson |
| Goat anti-Rabbit IgG (H + L) | ImmunoResearch | |
| anti-RFP antibody | 66 nM | ab65856, Abcam |
