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. 2017 Feb 1;113(3):321–331. doi: 10.1093/cvr/cvx002

Figure 6.

Figure 6

Myocyte relaxation is unaltered by nNOS.eGFP expression. (A) Returning velocity (dl/dt, μm/s) is faster in the presence of ISO in both nNOS.eGFP and eGFP overexpressing LV myocytes in the presence or absence of L-NAME (**P < 0.01; ****P < 0.0001 vs. basal, n = 11–19 cells from four hearts; two-way ANOVA) but is unaltered by nNOS.eGFP expression (P = NS vs. eGFP; two-way ANOVA) or by L-NAME (P = NS, n = 8–12 cells from three hearts per group; two-way ANOVA). (B) Similarly, the rate of decay of the [Ca2+]i transients (Tau1) was significantly faster in the presence of ISO in both groups (n = 10–18 cells from four hearts per group; two-ANOVA, **** P < 0.0001 vs. basal) but did not differ between nNOS.eGFP and eGFP overexpressing LV myocytes (P = NS, n = 10–18 cells from four hearts per group; two-way ANOVA). (C) Western blotting showed no significant differences in total and serine 16-phosphorylated PLB between eGFP and nNOS.eGFP expressing hearts (P = NS, n = 4 per group, Mann-Whitney test). Molecular weight (shown on the right side of the immunoblots) is expressed in Kilo Daltons.