Figure 5.

Tipifarnib depolymerizes actin and increases eNOS expression in HPAECs and mouse lungs. (A) Graph and (B) representative western blot show changes in globular/filamentous (G/F) actin ratio in HPAECs treated with tipifarnib (0.1 µmol/L) for 2–24 h, as indicated. n = 7–8. (C) eNOS mRNA and (D, E) eNOS protein expression in cells stimulated with tipifarnib; n = 5–6. (F, G) eNOS protein expression in the lungs of mice exposed to normoxia or chronic hypoxia and treated with either vehicle or tipifarnib, n = 6. Results are expressed as fold- change of controls; Data represent mean ± SEM *P < 0.05 vs. normoxic control. 1-way ANOVA with Tukey post-test.