Fig. 4. Western blot probed with hepatitis E virus (HEV) 110-610-based ELISA negative human sera from this study (lanes 1-7). Arrow indicates the 54.6 kDa protein of interest. There are some aggregates and degraded products. Five HEV negative human sera previously characterized by commercial kit were used as control for western blot specificity (data not shown). M, protein marker.