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. 2017 Mar 27;127(5):1646–1650. doi: 10.1172/JCI85504

Figure 3. Lentiviral manipulation of Ephexin5 in the DG ameliorates learning and memory deficits in developing hAPP mice.

Figure 3

(A) Timeline for the injection of WT and AD mice with lentivirus. (B) Representative immunofluorescence staining of the DG from a mouse injected with lentivirus. Sections were stained for GFP and labeled with DAPI. Scale bars: 10 μm. (C) Representative Western blot and quantification of actin-normalized Ephexin5 levels in microdissected hippocampi from mice injected with lentivirus expressing shRNA against Ephexin5 (E5) or scrambled hairpin (Scr). n = 7 mice. (D) NPP testing of object investigation for 3-month-old mice. Shown is the percentage time spent investigating each object, calculated as described in the legend for Figure 2. WT-Scr (n = 8), hAPP-Scr (n = 8), hAPP-E5 (n = 10), WT-E5 (n = 10). (C and D) *P < 0.05 and **P < 0.01. For comparison of lentivirus-injected hippocampal Ephexin5 levels, a paired t test was performed (C). Analysis of the NPP test was performed by 1-way ANOVA with a Kruskal-Wallis correction, since the data were not normally distributed (D).