Figure 2.

The SLC4A7 BP-associated locus shows allele-dependent DNA-nuclear protein interactions. (A) EMSA with labelled-probes corresponding to both alleles of the 10 highest priority SNPs at SLC4A7. The start codon NEK10 appears on the right or of the figure. Unbound probes as visualized at bottom of autoradiograph. Representative figure of n=3 replicates. (B) EMSA comparing VEC and VSMC nuclear extracts for three SNPs shows the shift present in VSMC nuclear extracts virtually absent with VEC nuclear extracts. Representative figure of n=2 replicates. All lanes had 10 femtomoles of labelled double-stranded oligonucleotide probe with 10 ng of corresponding nuclear extracts.