Fig. 1. Suppressive effect of JS-III-49 on the production of inflammatory mediators.

(A) Chemical structure of JS-III-49. (B) RAW264.7 cells pretreated with the indicated doses of (left panel) JS-III-49 or (right panel) L-NAME for 30 min were treated with LPS (1 µg/ml) for 24 h, and NO levels in the cell culture media were determined. (C) RAW264.7 cells pretreated with the indicated dose of (left panel) JS-III-49 or (right panel) indomethacin (Indo) for 30 min were treated with LPS (1 µg/ml) for 24 h, and PGE₂ levels in the cell culture medium were determined by EIA. (D) RAW264.7 cells were treated with the indicated dose of JS-III-49 for 24 h, and the cell viability was measured by MTT assay. ^*p<0.05, ^**p<0.01 compared to control.