Figure 3.

DNA methylation and histone H3K9me3 enrichment does not correlate with DUX4p copy number. A-C. Schematic view of isogenic muscle cells carrying 1 (A), 5 (B), or 12 (C) DUX4p repeats and an EGFP reporter gene, used for MeDIP and ChIP analyses. D-I. Isogenic muscle cells differing for DUX4p copy-number were analyzed by MeDIP for DNA methylation (D-F) and by ChIP for H3K9me3 (G-I), on DUX4p (D, G), on negative (E, H) or positive (F, I) control regions, respectively, Zeocine gene (E, H) and Major Satellite repeats (F, I). ChIP-qPCR data are shown relative to total H3. The mean of the signals obtained from three independent experiments is shown (D-F, G-I). The error bars represent SEM of independent biological replicates.