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. 2016 Dec 28;26(4):753–767. doi: 10.1093/hmg/ddw426

Figure 6.

Figure 6

PRC1 is required to maintain the endogenous DUX4 gene repressed in human primary muscle cells. A. RT-qPCR of BMI1 expression in human primary myotubes from healthy donor, transfected with siRNA targeting BMI1 (siBMI1) or a non-targeting control (siNT). B. RT-qPCR showing selective DUX4 de-repression upon BMI1 depletion (siBMI1), in the same cells in (A). C. RT-qPCR of DYSTROPHIN (DYS) expression, showing no significant alteration of muscle differentiation, in the same cells in (A). BMI1, DUX4 and DYS levels are relative to GAPDH, used as normalizator. Error bars represent SEM of four independent experiments. Asterisks indicate statistical significance (P value) as evaluated by Student’s t-test. ***= P < 0.001; * P < 0.05; ns = non significant.