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. 2017 Apr 28;12(4):e0176702. doi: 10.1371/journal.pone.0176702

Fig 2. Heterologous complementation with S. cerevisiae rtf1 restores wild-type phenotype in the A. fumigatus rtfA mutant.

Fig 2

Aspergillus fumigatus ΔrtfA strain (TRRM3) was transformed with plasmid pRRM4, which contains rtf1 from S. cerevisiae. (A) Effective complementation with rtf1 was confirmed by diagnostic PCR analysis using primers Scer_rtf1Afum_rtfA_f and Scer_rtf1_Afum_rtfA_r. Plasmid pRRM4 was used as a positive control, yielding the expected 1.7 kb PCR product. Aspergillus fumigatus ΔrtfA strain was used as negative control. (B) A. fumigatus strains WT (CEA10), ΔrtfA (TRRM4), and com-rtf1 (TRRM7) were point-inoculated on GMM and incubated at 37°C. (C) Colony diameters were measured after three days of growth. Values represent the average of three replicates. Different letters above the bars indicate significantly different values (p ≤ 0.05). Error bars represent standard error.