Fig 3. rtfA is a repressor of conidiation in A. fumigatus.

(A) Wild type (WT), ΔrtfA, complementation (com), and overexpression rtfA (OErtfA) strains were grown in liquid stationary cultures and whole mycelial mats were collected at 48 h and 72 h, homogenized, and conidia were quantified with a hemocytometer. Expression analysis of brlA (B) and wetA (C), two key genes in the conidiation regulatory pathway, at 48 h and 72 h. (D) Micrographs showing conidiation in the ΔrtfA strain when grown in liquid shaking cultures. Cultures were grown at 37°C for 72 h at 250 rpm. Scale bars represent 100 μm in the 10x micrographs and 10 μm in the 40x micrographs.