Fig 5. Peripheral Htt silencing does not rescue striatal transcriptional dysregulation in Htt^Q111/+ mice.

Using both RNAseq (A) and QRT-PCR (B), we assessed transcriptional markers of HD-like pathology in the striatum. Four transcripts, Drd1a, Pde10a, N4bp2, and Islr2 were selected to illustrate the agreement between QRT-PCR and RNAseq results. We successfully replicated HD-relevant, steady state transcriptional changes previously characterized in Htt^Q111/+ and wild-type mice, but Htt ASO treatment failed to rescue these phenotypes. ‡ p ≤ 0.05, ‡‡ p ≤ 0.01, ‡‡‡ p ≤ 0.001: by factorial ANOVA § FDR ≤ 0.05, §§ FDR ≤ 0.01, §§§ FDR ≤ 0.001 Abbreviations: counts per million (CPM).