Figure 3. Identification of cells producing TNF-α and IFN-γ in the inflamed colon tissue.

Lamina propria cells were isolated from the colons of DSS-treated CD300f^+/+ or CD300f^–/– mice, collected on day 7 (A and C) or day 14 (B and D). The intracellular expression of TNF-α and IFN-γ was determined by flow cytometry in the following cell populations: macrophages (CD45⁺F4/80⁺CD11b⁺CD14⁺Ly6G^–CD11c^–), DCs (CD45⁺CD11c⁺F4/80^–Ly6G^–CD64^–), neutrophils (CD45⁺CD11b⁺Ly6G⁺CD11c^–F4/80^–), mast cells (CD45⁺CD11b⁺FcεRI⁺), T cells (CD45⁺CD3⁺), B cells (CD45⁺CD3^–CD19⁺), NK cells (CD45⁺CD3^–NK1.1⁺), and NKT cells (CD45⁺CD3⁺NK1.1⁺). The graphs show the percentages (left) and total numbers (right) of cells expressing TNF-α (A and B) or IFN-γ (C and D). Data are expressed as means + SEM (n = 3–8, each group). Two-tailed paired Student’s t test was used to determine statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001).