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. 2017 Apr 17;127(5):1905–1917. doi: 10.1172/JCI89531

Figure 5. CD300f-deficient BMDCs exacerbate inflammation and delay resolution of inflammation in colonic inflammation.

Figure 5

(A) Purified, CFSE-labeled CD11c+ BMDCs (2 × 106 cells) derived from CD300f+/+ or CD300f–/– mice were injected i.v. into CD300f+/+ mice on day 4 during DSS administration. The images illustrate the presence of the transferred, CFSE+ BMDCs in the colon tissues; scale bars: 20 μm. (B) DAI scored during and after DSS administration; BMDC transfer time is indicated. (C–E) The colon length (C), macroscopic inflammation score (D), and microscopic inflammation score (E) were determined on day 7 (Inflammation) and day 14 (Resolution). The images in E are representative images of H&E-stained colon tissues; scale bars: 500 μm. (F) Colon tissue lysates were evaluated for the indicated cytokine levels. All graphs show mean values; error bars indicate SEM (n = 4, each group). Two-tailed paired Student’s t test was used to determine statistical significance (*P < 0.05, **P < 0.01).