Component	Volume per reaction (µl)	Final concentration
2x RT-PCR Buffer*	12.5	1x
Nuclease-free water*	1.08	n/a
Detection Enhancer*	1.67	n/a
Cog 1F (10 µM)	1	400 nM
Cog 1R (10 µM)	1	400 nM
Ring 1E-probe (10 µM)	0.5	200 nM
Cog 2F (10 µM)	1	400 nM
Cog 2R (10 µM)	1	400 nM
Ring 2-probe (10 µM)	0.5	200 nM
MS2F (10 µM)	0.25	100 nM
MS2R (10 µM)	0.25	100 nM
MS2P-probe (10 µM)	0.25	100 nM
2x RT-PCR enzyme *	1	1x
Master Mix volume	22
* included in the real time RT-PCR kit