Component	Final concentration	vol/rxn (µl)
5x RT-PCR buffer	1x	5.00
dNTP mix (10 mM)	0.4 mM	1.00
Enzyme mix (RT and Taq)		1.00
Forward primera	0.5 µM	0.50
Reverse primer a	0.5 µM	0.50
Rnase inhibitor (20 U/µl)	20 U	1.00
Rnase-free water		11.00
Total Volume		20.00
aforward and reverse primer sets for GI and GII group are Cog1F + G1SKR, and Cog2F + G2SKR, respectively.
Second round RT-PCR
Component	Final concentration	vol/rxn (µl)
5x RT-PCR buffer	1x	5.00
dNTP mix (10 mM)	0.4 mM	1.00
Enzyme mix (RT and Taq)		1.00
Forward primerb	0.5 µM	0.50
Reverse primerb	0.5 µM	0.50
Rnase inhibitor (20 U/µl)	20 U	1.00
Rnase-free water		14.00
Total Volume		23.0
bforward and reverse primer sets for GI and GII group are G1SKF + G1SKR, and Ring2 primer + G2SKR, respectively.
Note: above informaion was slightly modified from the original article19