Chemical	Final Concentration	Amount
5x M9 salts	1x	20 mL
1 M MgSO4	2 mM	200 μL
1 M CaCl2	0.1 mM	10 μL
20% glucose	0.40%	2 mL
2% biotin-free Casamino acid	0.02%	1 mL
DI Water	N/A	76.8 mL