| Step 1 | Step 2 | Step 3 | Step 4 | Step 5 | Step 6 | Step 7 | Step 8 | Step 9 |
| 98°C | 98°C | 70°C | 72°C | 98°C | 60°C | 72°C | 72°C | 4°C |
| 0:30 | 0:10 | 0:30 | 01:00/kb | 0:10 | 0:30 | 01:00/kb | 2:00 | ∞ |
| Repeat 12 times | Repeat 25 times | |||||||
| -1°C / cycle |
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