| Name | Volume |
| Cell Lysate (template) | 10 μL |
| Primer (each) | 0.625 μL (each) |
| 5x Q5 buffer | 5 μL |
| Q5 Polymerase | 0.25 μL |
| dNTP Mix | 0.5 μL |
| DI Water | 6.75 μL |
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