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. 2017 Mar 9;(121):55300. doi: 10.3791/55300
Name Concentration Notes
CaCl2 100 mM
Carbeniccilin 50 mg/mL (1000x)
DTB 50 μg/mL
IPTG 0.5 M
SMCC 20 mg/mL 2 mg into 100uL DMSO
SPDP 20 mg/mL 2 mg into 100uL DMSO
LC-LC-biotin 20 mg/mL 2 mg into 100uL DMSO
HRP 10 mg/mL In PBS
SA (indirect) 10 mg/mL In PBS
SA (direct) 0.17 μg/mL In PBS
BSA 20 mg/mL In PBS
DTT 100mM In DI Water
EDTA 5 mM 18.6 mg into 10 mL PBS
Casein 0.50% In PBS
Tween 80 20% In DI Water
Tween 80 in PBS 0.05%
Sodium Acetate 50 mM In DI Water, Adjust to 5.1 pH using 3 M HCl
TMB 1% In DMSO
H2O2 3% In DI Water
H2SO4 2 M In DI Water