Name | Concentration | Notes |
CaCl2 | 100 mM | |
Carbeniccilin | 50 mg/mL (1000x) | |
DTB | 50 μg/mL | |
IPTG | 0.5 M | |
SMCC | 20 mg/mL | 2 mg into 100uL DMSO |
SPDP | 20 mg/mL | 2 mg into 100uL DMSO |
LC-LC-biotin | 20 mg/mL | 2 mg into 100uL DMSO |
HRP | 10 mg/mL | In PBS |
SA (indirect) | 10 mg/mL | In PBS |
SA (direct) | 0.17 μg/mL | In PBS |
BSA | 20 mg/mL | In PBS |
DTT | 100mM | In DI Water |
EDTA | 5 mM | 18.6 mg into 10 mL PBS |
Casein | 0.50% | In PBS |
Tween 80 | 20% | In DI Water |
Tween 80 in PBS | 0.05% | |
Sodium Acetate | 50 mM | In DI Water, Adjust to 5.1 pH using 3 M HCl |
TMB | 1% | In DMSO |
H2O2 | 3% | In DI Water |
H2SO4 | 2 M | In DI Water |