Figure 1.

The human NLRP3 3′-UTR is alternatively polyadenylated and the isoforms are differentially expressed. a, the human NLRP3 3′-UTR visualized in the UCSC genome browser (hg19) with ESTs, polyadenylation sites from PolyA_DB, and mammalian conservation tracks. Location of AU-rich elements (AUUUA pentamers) are indicated. b, 3′-RACE of the NLRP3 3′-UTR in CD14⁺ monocytes or M-CSF-differentiated macrophages. PCR products were analyzed by agarose gel electrophoresis. c–e, expression of the short and long 3′-UTR were quantified in the UCSC genome browser for expression tracks sourced from the ENCODE consortium (CSHL) (c), NIH Roadmap Epigenomics project (d), or GTEx project (e). The ratio for individual samples is plotted. f, qPCR analysis of 3′-UTR isoform expression in primary CD14⁺ monocytes or M-CSF-differentiated macrophages using primer pairs that amplify specifically within the short or long 3′-UTR isoforms. Isoform expression was normalized to ACTB and expression of representative samples relative to the short isoform is shown.