Figure 2.

Co-purification of TOC components with KOC1. A, sequential fractions of NTAP-KOC1 IgG-affinity purification were analyzed by Western blotting. The membrane was probed with antibodies against KOC1, TOC159, TOC75, TOC33, TIC56, and LHCB2 (T, total; L, load; Ft, flow-through; W1, first wash; W5, last wash; TEV, eluate). 50 μg of proteins (total, load, flow-through, and first wash) or 10% of the fractions (last wash and eluate) were loaded. Several identical blots were used for immunoblotting. TAP:WT was used as a negative control. B, interaction networks of KOC1 using the list of co-immunopurified proteins and the STRING 10.0 program.