Skip to main content
. 2017 Mar 10;292(17):6952–6964. doi: 10.1074/jbc.M117.776468

Figure 3.

Figure 3.

KOC1 sequence analysis and mutant characterization. A and B, the amino acid sequence of KOC1 contains a kinase domain (orange; amino acids (aa) 39–306). Underlined amino acids 45–53 are conserved in the catalytic domain; amino acid 74 is predicted to bind ATP. In the HERC2-related region (blue; amino acids 376–531), underlined amino acids share homology with the KEG protein. A predicted transmembrane stretch (TM; amino acids 549–572) is highlighted in red. C, images of 2-week-old WT, koc1-1, koc1-2, and NTAP-KOC1:koc1-1 plants grown in vitro. D, total protein extracts of 2-week-old plants (WT, koc1-1, koc1-2, and NTAP-KOC1) were separated by SDS-PAGE and transferred to nitrocellulose membrane. Antibodies against TOC159A, TOC75, TOC120, KOC1, TOC33, and TIC110 were used. Actin was used as a loading control. *, bands corresponding to overexpressed NTAP-KOC1.