Figure 7.

Overexpression of TbHrg-V5 leads to accumulation of heme in BS cells and switch of procyclins in PC cells. A, dynamic accumulations of radioactive ¹⁴C-heme in WT BS cells (black line) and in those overexpressing TbHrg-V5 (gray line). Cells incubated with ¹⁴C-heme were quantified by scintillation. B, Western blot analysis of wild type (WT) PC cells and those overexpressing V5-tagged TbHrg, non-induced (−tet), and RNAi-induced cells (+tet, 24 h). Their lysates were immunodecorated with antibodies against the early GPEET procyclin and the late EP1 procyclin as well as with α-tubulin antibodies, used as a loading control. The overexpression of TbHrg-V5 was monitored with α-V5 antibodies. Statistical significance for GPEET (n = 3) and EP1 (n = 2) protein expression (WT value set to 100%) was determined by Student's t test (*, p ≤ 0.05; **, p ≤ 0.01, ***, p ≤ 0.001).