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Decamer stabilization of cytoplasmic 2-Cys Prx from Leishmania and some distantly related homologues is Ca2+-independent. SEC chromatograms of air-oxidized LbPrx1m at 130 μm (a), cytoplasmic Prx1 from L. braziliensis (LbPrx1a) at 43 μm (b), human Prx2 at 130 μm (c), and yeast TSA1 at 43 μm (d) loaded (2 ml) into a HiLoad Superdex 200 16/600 column pre-equilibrated with Tris buffer (pH 7.5) containing 25 mm CaCl2 or 5 mm EDTA. mAU, milliabsorbance units.
