Table 2.

ITC measurement of HMP-1 binding to HMP-2, vinculin D1, and DEB-1 D1

Proteins		KD	ΔH	TΔS	ΔG
		nm	kcal mol−1
HMP-1 + HMP-2
HMP-1(FL)	HMP-2(13-678)	1	−21.5	−9.2	−12.3
HMP-1(FL)	HMP-2(13-678)·pHMR-1(cyto80)	3.9	−22.9	−11.3	−11.6
HMP-1 + vinculin
HMP-1 M	Vinculin D1	600	18.5	27.0	−8.5
HMP-1 MI-MII	Vinculin D1	572	13.1	21.6	−8.5
HMP-1 MI	Vinculin D1	3.1	7.6	19.2	−11.6
HMP-1 + DEB-1
HMP-1 M	DEB-1 D1	NDa	ND	ND	ND
HMP-1 MI-MII	DEB-1 D1	ND	ND	ND	ND
HMP-1 MI	DEB-1 D1	150	−21.8	12.5	−9.3

^a ND, not determined.