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A, SDS-PAGE analysis of recombinant BafX, BafY, BafZ, Orf2, and Orf3 purified from E. coli BL21 (DE3). B, the re-annotation of the coding sequence of Orf3. Note: the black and green letters denote partial C-terminal coding sequence of Orf2 and partial N-terminal coding sequence of Orf3, respectively. The stop codon of Orf2 is marked in red. The underlined GTG codons in green and blue represent the corrected and the previously mis-annotated start codons of Orf3, respectively.
