Figure 2.

L-OPA1 alone has the capacity to elongate mitochondria in pro-fusion conditions. Mitochondrial tubule formation was assessed in normal conditions (A) and conditions of fission inhibition (B) and SIMH (C and D) in cells expressing different OPA1 variants. Mitochondrial lengths were categorized into long tubules, intermediate, and fragmented. In both fission inhibition and SIMH conditions, OPA1-v1 and v1ΔS1 significantly increased the formation of tubular mitochondria. Mitochondrial tubule formation by OPA1-v5 was minimal. n = 4. Error bars are S.E.