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. 2017 Apr 14;13(4):e1006721. doi: 10.1371/journal.pgen.1006721

Fig 4. Rng8 and Rng9 have roles beyond that of regulating Myo51 motor.

Fig 4

(A) Cross of wildtype h- expressing Myo52-tdTomato with h+ wildtype, rng8Δ or rng9Δ strains expressing Myo51-3YFP. (B) Quantification of Myo51-3YFP intensity at the fusion site in wildtype, rng8Δ and rng9Δ strains as in (A), showing reduction of Myo51 levels in the mutants (N>11); (***) P < 5 × 10−4, t-test. (C) Localization of Myo52-tdTomato in homothallic wildtype and myo51Δ strains. (D-E) Measurements of Myo52-tdTomato zone width (D) and fluorescence intensity (E) at the cell-cell contact site in strains as in (C) (N = 15). (F) Quantifications of the number of Myo52 dots observed in myo51Δ mutants in comparison to wildtype (N>100 cells). (G) Fusion efficiency of h90 wildtype, myo52Δ, myo52Δ rng8Δ, myo51Δ and myo51Δ rng8Δ strains on pad (N>200); (*) P < 0.04, (**) P < 0.003 t-test. (H) Localization of Rng8-mEGFP in myo51Δ and myo52Δ mutants during vegetative growth (top) and during mating (bottom) in h+ cells crossed to a wildtype h- strain expressing Myo52-tdTomato. (I) Measurements of average Rng8-mEGFP fluorescence at fusion site in strains as in H (N = 10). (J) Measurements of Rng8-mEGFP total zone width at the cell-cell contact site in strains as in (H) (N = 10); (***) P < 7 × 10−5, t-test. Bars, 2 μm.